polyprep microscope slides Search Results


polyprep microscope slide  (Millipore)
90
Millipore polyprep microscope slide
Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a <t>Polyprep</t> <t>microscope</t> slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope
Polyprep Microscope Slide, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyprep microscope slide/product/Millipore
Average 90 stars, based on 1 article reviews
polyprep microscope slide - by Bioz Stars, 2026-03
90/100 stars
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polyprep slides  (Millipore)
90
Millipore polyprep slides
Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a <t>Polyprep</t> <t>microscope</t> slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope
Polyprep Slides, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/polyprep slides/product/Millipore
Average 90 stars, based on 1 article reviews
polyprep slides - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
imager m1 microscope  (Carl Zeiss)
90
Carl Zeiss imager m1 microscope
Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a <t>Polyprep</t> <t>microscope</t> slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope
Imager M1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/imager m1 microscope/product/Carl Zeiss
Average 90 stars, based on 1 article reviews
imager m1 microscope - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier
poly-llysine-coated slides  (Millipore)
90
Millipore poly-llysine-coated slides
Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a <t>Polyprep</t> <t>microscope</t> slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope
Poly Llysine Coated Slides, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/poly-llysine-coated slides/product/Millipore
Average 90 stars, based on 1 article reviews
poly-llysine-coated slides - by Bioz Stars, 2026-03
90/100 stars
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fluoromount g  (SouthernBiotech)
97
SouthernBiotech fluoromount g
Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a <t>Polyprep</t> <t>microscope</t> slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope
Fluoromount G, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fluoromount g/product/SouthernBiotech
Average 97 stars, based on 1 article reviews
fluoromount g - by Bioz Stars, 2026-03
97/100 stars
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Image Search Results


Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a Polyprep microscope slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope

Journal: Cancer Immunology, Immunotherapy : CII

Article Title: Role of CD44 in lymphokine-activated killer cell-mediated killing of melanoma

doi: 10.1007/s00262-011-1105-4

Figure Lengend Snippet: Involvement of CD44 in the adhesion between IL-2-activated LAK cells and B16F10 melanoma. a CFSE-labeled LAK cells from CD44 WT and CD44 KO mice were cultured for 2 h at 37°C in plates containing adherent B16F10 tumor cells. LAK cells not adhering to B16F10 cells were removed by gently washing the cultures with medium. The percentage of adherent cells was quantified by measuring the level of fluorescence remaining following washing of the cells compared to the fluorescence in non-washed wells (total fluorescence). Asterisk indicates statistically significant difference when compared with the CD44 WT unstimulated splenocytes, P ≤ 0.05. b LAK cells were mixed with B16F10 and spun down at 400 rpm for 5 min and then incubated at 37°C for 15 min. The mixed cells were resuspended gently, and 60 μl was incubated on a Polyprep microscope slide for 15 min. The slides were stained with Cy5-conjugated anti-CD44 (IM7) for 1 h and analyzed using a confocal microscope

Article Snippet: The mixed cells were resuspended gently, and 60 μl was incubated on a Polyprep microscope slide (Sigma-Aldrich, St. Louis, MO) for 15 min at 37°C.

Techniques: Labeling, Cell Culture, Fluorescence, Incubation, Microscopy, Staining